Abstract
Geometrical isomers of methyl 9,12-octadecadienoate and methyl 9,12,15-octadecatrienoate were fractionated by thin-layer chromatography on Silica Gel G modified with silver nitrate and ammonium hydroxide. Dienoic esters were completely separated into three classes: (1) all trans; (2) trans-cis and cis-trans; and (3) all cis. Trienoic esters were similarly separated into four classes: (1) all trans; (2) di- trans, mono- cis; (3) di- cis, mono- trans; and (4) all cis. Complete separation of the cis, trans isomers of methyl octadecadienoate and of the mono- cis, di- trans and mono- trans, di- cis isomers of methyl octadecatrienoate was achieved by gas-liquid chromatography on glass capillary columns (Apiezon L).
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