Abstract
Thirty-one photosynthetic pigments (chlorophylls, carotenoids and degradation products) from the seaweed, Caulerpa prolifera (chlorophyta), Jania rubens (rhodophyta) and Padina pavonica (phaeophyta), were separated in a single-step procedure by reversed-phase high-performance liquid chromatography, using an elution gradient of methanol, acetone and ammonium acetate solution, and a program time of 65 min, to obtain high resolution peaks of the separated photosynthetic pigments. We consider that the program times used make it possible to inject a mixed extract of the different seaweed groups. Eighteen photosynthetic pigments were separated from Caulerpa prolifera, 16 from Jania rubens and 14 from Padina pavonica. Chlorophyll b, micronone, microxanthin, neoxanthin, siphonein and siphonoxanthin were the most typical and characteristic pigments of Caulerpa prolifera, while chlorophyll c 1, c 2, fucoxanthin, fucoxanthol, flavoxanthin, diatoxanthin were the most typical pigments in Padina pavonica. In Jania rubens, on the other hand, chlorophyll d, α-cryptoxanthin, β-cryptoxanthin and fucoxanthin were the most common pigments.
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