Abstract
In order to enrich and separate three coumarins (columbianetin acetate, osthole and columbianadin) from Angelicae Pubescentis Radix (APR), an efficient method was established by combining macroporous resins (MARs) with preparative high-performance liquid chromatography (PHPLC). Five different macroporous resins (D101, AB-8, DA-201, HP-20 and GDX-201) were used to assess the adsorption and desorption characteristics of three coumarins. The result demonstrated that HP-20 resin possessed the best adsorption and desorption capacities for these three coumarins. Moreover, the adsorption dynamics profiles of three coumarins were well fitted to the pseudo second order equation (R2 > 0.99) for the HP-20 resin. The adsorption process was described by the three isotherms models including Langmuir (R2 > 0.98, 0.046 ≤ RL ≤ 0.103), Freundlich (R2 > 0.99, 0.2748 ≤ 1/n ≤ 0.3103) and Dubinin Radushkevich (R2 > 0.97). The contents of columbianetin acetate, osthole and columbianadin in the product were increased 10.69-fold, 19.98-fold and 19.68-fold after enrichment, respectively. Three coumarins were further purified by PHPLC and the purities of them reached above 98%. Additionally, the anti-inflammatory effects of these three coumarins were assessed by Lipopolysaccharide (LPS)-induced RAW 264.7 cells. It was found that the production of NO and MCP-1 was obviously inhibited by three coumarins. Columbianetin acetate, osthole and columbianadin could be used as potentially natural anti-inflammatory ingredients in pharmaceutical products. It was concluded that the new method combining MARs with PHPLC was efficient and economical for enlarging scale separation and enrichment of columbianetin acetate, osthole and columbianadin with anti-inflammatory effect from the APR extract.
Highlights
Angelicae Pubescentis Radix (APR) derived from the roots of Angelica pubescens Maxim.f.biserrata Shan et Yuan has been recorded in Chinese pharmacopoeia and is usually used to treat rheumatic arthralgia [1]
It has not been reported that three major coumarin compounds were enriched and separated adsorption resins, and the desorption ratio of the three adsorption resins were 85.72%, 90.84% and by macroporous resins from APR extract
A simple and useful method was established for simultaneous enrichment and separation of columbianetin acetate (CBA), OE and CBD from APR extract using macroporous resins (MARs) coupled with preparative high-performance liquid chromatography (PHPLC)
Summary
Angelicae Pubescentis Radix (APR) derived from the roots of Angelica pubescens Maxim.f.biserrata Shan et Yuan has been recorded in Chinese pharmacopoeia and is usually used to treat rheumatic arthralgia [1]. 70 coumarins including columbianetin, columbianadin (CBD), osthole (OE), columbianetin acetate (CBA), isoimperatorin, bergapten and columbianetin-β-d-glucopyranoside have been isolated from. Molecules 2019, 24, 2664; doi:10.3390/molecules24142664 www.mdpi.com/journal/molecules large-scale industrial production due to several drawbacks, including inefficiency, high cost, and poor purity. Macroporous resins (MARs) have been extensively used to separate and purify pharmacologically-active natural products, including flavonoids, phenolic, glycosides, saponins due to high adsorption capacity, environmentally-friendly and easy regeneration properties [13,14,15,16,17,18,19]. Molecules 2019, 24, 2664 enriched and separated by macroporous resins from APR extract. The purpose of the current study was to develop a quick and efficient method for separation and enrichment of CBA, and CBD extract.
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