Abstract
A method is presented for the separation and detection of eight synthetic corticosteroids in bovine liver tissues. Following sample preparation, the samples were separated by isocratic LC (mobile phase: 85% methanol, 15% dichloromethane) using a porous graphite (PGC) column and detected by ion trap MS-MS, using an atmospheric pressure chemical ionization (APCI) source and monitoring positive ions. An octadecyl-silica phase system was also used for comparison. With the PGC stationary phase the critical isomer separation of dexamethasone and betamethasone was achieved. The performances of MS-MS, MS3, selected ion monitoring (SIM) and selected reaction monitoring (SRM) detection modes were checked and best conditions of sensitivity were obtained in MS-MS mode.
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