Abstract
The free hydroxyl groups of saccharides and sugar alcohols react with phenyl isocyanate to yield very stable and strongly UV-absorbing derivatives, which possess good chromatographic properties in a reversed-phase system. UV monitoring at ca. 240 nm allows detection down to the 0.5–10 ng level.Reducing sugars yield a peak for each enantiomer, while non-reducing sugars and sugar alcohols yield a single peak. The chromatograms resemble those obtained by gas-liquid chromatography after silylation.Oligomers containing eight glucose units have been separated with good resolution by this method. Applications to the separation and determination of sugar alcohols and wood hydrolysates are described.
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