Separation and determination of 4-(1,1,3,3-tetramethylbutyl)phenyl dihydrogenphosphate and di-[4-(1,1,3,3-tetramethylbutyl)phenyl] hydrogenphosphate by high-performance liquid chromatography

Abstract

4-(1,1,3,3-Tetramethylbutyl)phenyl dihydrogenphosphate and di-[4-(1,1,3,3-tetramethylbutyl)phenyl] hydrogenphosphate (0.01–1 mg ml -1) in aqueous phosphoric acid raffinates can be separated on a reversed-phase μ Bondapak C 18 column by gradient elution with methanol/water, and quantified at 267 nm. Raffinates are extracted with 4-methyl-2-pentanone; the two phosphates can then be determined with errors less than ±5%.