Abstract
ABSTRACT A high performance liquid chromatography (HPLC) method has been developed for the separation and quantitation of clavulanic acid (CA), sulbactam (SUL), and tazobactam (TAZ) in human sera. The optimum mobile phase used in conjunction with a β-cyclodextrin (β-CyD) column contained methanol-5 mM tetraethylammonium acetate (TEAA) (35 : 65, v/v, pH 4.5) and 1.0 mL/min flow rate for chromatographic separation. The mean recoveries of CA, SUL, and TAZ range from 84.4 to 87.8%, 88.4 to 90.6%, and 89.0 to 90.8% respectively, from human serum samples spiked with the inhibitors, are routinely obtained. The lower limit of quantitation ( < 0.156 µg/mL) and the low coefficient of variation ( < 3.92%) assure that this method is sensitive and reproducible.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Journal of Liquid Chromatography & Related Technologies
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
