Abstract
A method is described in which twenty water-soluble acid dyes, isolated from food products by means of DEAE-Sephadex, were detected by thin-layer chromatography using polyamide plates.The isolation procedure involved shaking samples with the Sephadex and then eluting the dyes by column chromatography. In polyamide thin-layer chromatography, the solvent system suitable for the separation of the dyes was pyridine—methanol—28% aqueous ammonia—water (5:6:1:16).This is a simple and rapid method for the detection of acid dyes in cellulosic and proteinaceous samples, especially when the dyes are present in low concentrations.
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