Abstract
AbstractPolygalacturonase (PG) produced by Colletotrichum musae in liquid culture on banana pectic substances as the sole carbon source resolved into five distinct isozymes, with isoelectric points ranging between pl 4.8‐6.5. All five isozymes were detected in banana tissues rotted by C. musae.Isozymes PG I (90 kD) and PG II (36 kD) were separated from PG III, IV and V (60‐70 kD) by gel filtration on a Superose 12 column. PG III, IV and V were resolved on a hydrophobic column (Alkyl Superose, HR 5/5) by changing gradients of 0.1 M sodium acetate buffer (pH 5.0). PG II, III, IV and V had endo‐activity and PG I had exoactivity.
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