Abstract
Antarctic krill is the biggest fishery resource in the world with high content of phosphatidylcholine (PC) as a valuable natural product. A new method was developed to extract and separate Antarctic krill PC. The PC was extracted only by acetone from Antarctic krill oil and separated by silica gel column chromatography mixtures of chloroform and methanol as eluting solvents. Thin-layer chromatography, high-performance liquid chromatography, and Fourier-transform infrared spectroscopy analyses were used to characterize the acetone-soluble Antarctic krill PC. The purity of the purified PC was able to reach 97.77 ± 0.65 %, which provided a reference basis for the preparation of standard Antarctic krill PC. Moreover, it was found in the fatty acid analysis that the purified PC contained mainly five fatty acids, including palmitic acid (41.25 %), oleic acid (8.62 %), 7-hexadecenoic acid (4.60 %), EPA (31.34 %), and DHA (14.52 %). The result is helpful for the analysis of Antarctic krill PC’s molecular structures and the evaluation of its nutritional value.
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