Abstract
Two active factors have been separated from the CNS of the crab that produce opposite affects on rates of water influx in crustaceans. The two factors are differentially soluble in water and acetone, and the water-soluble factor was partly purified by column chromatography and found to be a low molecular weight compound. When injected into crabs and crayfish held in hypotonic medium, the water-soluble fraction caused a decrease in 3H 2O influx, while the acetone-soluble fraction increased the influx rate. Neither of these factors affected sodium influx rates. It is suggested that these active factors play a role in influencing crustacean osmoregulation, and their neuroendocrine significance is discussed.
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