Abstract
This research was aimed to separate and analyse Monascus yellow pigment (MYP) with chromatography. MYP was in a mixture with red and orange pigments. MYP separation was performed in a silica gel column with eluent of ethyl acetate: ethanol: water = 90: 25: 4. The separated MYP were then subjected to analysis by using spectrophotometer, TLC, LC-PDA and LC-MS/MS. The results revealed that the separated MYP has a maximum absorption at 386 nm. Out of five MYP compounds detected by using LC-MS/MS, two of them were well known yellow pigment compounds, monascin and ankaflavin. Monascin and yellow II were the major pigment compounds in MYP.
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