Abstract
An anion-exchange HPLC method coupled with conductimetric detection was developed for the analysis of UDP-sugars, nucleotides, and sugar phosphates, each in a single chromatographic run. The analysis was applicable to concentrations over 50 pmol. The utility of this technique was demonstrated by the measurement of UDP-sugar 4′-epimerase activity of cell-free extracts from wild-type and mutant Neisseria meningitidis serogroup B strains, Additionally, the method has been applied to the analysis of the intermediates of glycolysis in human erythrocytes.
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