Separate and Simultaneous Effects of Silver Nanoparticles and Arsenic on the Taxonomic Diversity of Free-Living Marine Nematodes

Abstract

The effects of silver nanoparticles and arsenic at community levels have rarely been assessed in laboratory experiments, despite their obvious advantage in reflecting better the natural conditions compared to traditionally single species-focused toxicological experiments. In the current study, the multifaceted effects of these xenobiotics, acting alone or combined, on meiobenthic nematodes were tested in a laboratory experiment carried out in microcosms. The nematofauna was exposed to two concentrations (0.1 and 1 mg·L−1) of silver nanoparticles (Ag1/Ag2) and arsenic (As1/As2), as well as to a mixture of both compounds, for 30 days. The results particularly highlighted a significant decrease in the abundance and taxonomic diversity of nematodes directly with increasing dosages of these compounds when added alone at the highest concentration. The addition of these levels of xenobiotics seems to make the sediment matrix gluey, hence inducing greater mortality among microvores and diatoms feeders. Moreover, the nematofauna went through a strong restructuring phase following the exposure to both compounds when added alone, leading to the disappearance of sensitive taxa and their replacement with more tolerant ones. However, the similarity in nematofauna composition between control and mixtures of silver nanoparticles and arsenic (except for Ag1As2) suggests that the toxicity of the latter pollutant could be attenuated by its physical bonding to the former.