Abstract
Concentrates of urinary gonadotropin (fraction B) of men, postmenopausal women and eunuchs were applied to carboxymethylcellulose (CMC) columns in 0.01M ammonium acetate solution at pH 6.0. A nonretained fraction was obtained and then the material on the column was eluted with 0.5M ammonium acetate to yield a retained fraction. All of the recovered follicle stimulating hormone (FSH) and from ½ to ⅛ of the recovered luteinizing hormone (LH) were in the nonretained fraction; the other ½ to ¼ of the recovered LH was in the retained fraction. The FSH/LH ratios of the nonretained fraction varied from 3 to 4, whereas the FSH/ LH ratio for the retained fraction varied from less than 0.23 to less than 0.05. Carboxymethylcellulose chromatography thus effected a substantial separation of FSH and LH. (Endocrinology 77: 1085, 1965)
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