Seonamhaeicola aphaedonensis gen. nov., sp. nov., a member of the family Flavobacteriaceae isolated from a tidal flat sediment

Abstract

A Gram-stain-negative, non-motile, rod-shaped bacterial strain, AH-M5(T), which was isolated from a tidal flat sediment at Aphae Island in South Korea, was characterized taxonomically. Strain AH-M5(T) grew optimally at 25 °C, at pH 7.0-8.0 and in presence of 2.0% (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences revealed that strain AH-M5(T) clustered coherently with the type strains of Mangrovimonas yunxiaonensis and Meridianimaribacter flavus, showing 93.4-94.3% sequence similarity. The novel strain exhibited 16S rRNA gene sequence similarity values of less than 93.4% to the type strains of other recognized species. Strain AH-M5(T) contained MK-6 as the predominant menaquinone and iso-C(15 : 1) G, iso-C(15 : 0), iso-C(17 : 0) 3-OH and summed feature 3 (C(16 : 1)ω7c and/or C(16 : 1)ω6c) as the major fatty acids. The polar lipid profile of strain AH-M5(T) containing phosphatidylethanolamine and one unidentified lipid as major components was differentiated from those of the type strains of Mangrovimonas yunxiaonensis and Meridianimaribacter flavus. The DNA G+C content of strain AH-M5(T) was 34.8 mol%. Differential phenotypic properties, together with the phylogenetic and chemotaxonomic data, demonstrated that strain AH-M5(T) is distinguished from Mangrovimonas yunxiaonensis and Meridianimaribacter flavus. On the basis of the data presented, strain AH-M5(T) is considered to represent a novel genus and species within the family Flavobacteriaceae, for which the name Seonamhaeicola aphaedonensis gen. nov., sp. nov. is proposed. The type strain of the type species is AH-M5(T) ( = KCTC 32578(T) = CECT 8487(T)).