Abstract
In this paper, a novel electrochemiluminescence (ECL) imaging sensor array was developed for determination of hydrogen peroxide (H 2O 2), which was based on Cu/Zn alloy galvanic cell generated ECL. In alkaline solution, Cu/Zn galvanic cell was formed because of corrosion effect, the galvanic cell could supply stable potential for ECL generation of luminol, and the weak ECL emission could be enhanced by H 2O 2. The galvanic cell sensor array was designed by putting Cu/Zn alloy in 96-well microtiter plates separately. The relative ECL intensity was proportional with the concentration of hydrogen peroxide in the range of 1.0 × 10 −6 to 1.0 × 10 −4 mol l −1 and the detection limit was 3.0 × 10 −7 mol l −1 (3 σ), the relative standard deviation (R.S.D.) for 11 parallel measurements of 1.0 × 10 −5 mol l −1 H 2O 2 was 4.0%.
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