Abstract
ObjectiveThe component allergens from sawtooth oak, which is a main cause of tree pollinosis in Korea, have not been extensively characterized except Que ac 1. This study was undertaken to characterize the allergenic components from sawtooth oak pollen and investigate the diagnostic values of each component allergen.MethodsTranscriptomic analysis was performed to identify the birch pollen allergen homologues from sawtooth oak pollen. Recombinant Que ac 1, 2, 3, 6, 7, and 8 were produced in an E. coli expression system. IgE reactivity to each allergen was examined by ImmunoCAP and ELISA using the sera of 50 Korean tree pollinosis patients.ResultsSix birch pollen allergen homologues were identified using transcriptome analysis, as follows: Que ac 1 (54.8% identity to Bet v 1), Que ac 2 (79.7% to Bet v 2), Que ac 3 (24.9% to Bet v 3), 6 (71.3% to Bet v 6), Que ac 7 (80.9% to Bet v 7), and Que ac 8 (78.9% to Bet v 8). Que ac 1 sIgE was the most frequently recognized (84.0%), followed by Que ac 2 (12.0%), Que ac 3 (6.0%), and three other allergens (2.0% each). Que ac 1 was a dominant allergen affecting 83.7% of patients suffering from allergic rhinoconjunctivitis, and 92.9% of pollen food allergy syndrome patients.ConclusionFive novel IgE reactive components of sawtooth oak were characterized using transcriptome analysis. Que ac 1 is the single most important component allergen of sawtooth oak pollen.
Highlights
Oak is the most important cause of seasonal pollinosis in Korea
IgE reactivity to Que ac 1 was even stronger than IgE reactivity to Q. alba (t7) pollen extract, which is currently used for the diagnosis of oak allergy (Figure 1B)
We identified five new recombinant component allergens from sawtooth oak pollen in order to investigate the application of CRD in the diagnosis of oak allergy
Summary
Oak is the most important cause of seasonal pollinosis in Korea. The overall prevalence of allergic sensitization was 45.3%, and sensitization to oak was 6.6%, from 28,954 Korean adult patients in a retrospective study.[1]. Quercus acutissima, showed the strongest allergenicity among the Korean oak species.[5] Que ac 1, which is homologous to Bet v 1, was the most potent allergen from sawtooth oak pollen. This molecule (Que ac 1) was recognized by IgE antibodies from 91.3% of the oak pollinosis patients.[6] we believe that there are more allergen molecules that have yet to be identified. Approved by the Institutional Review Board of Severance Hospital (4–2017–1197)
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