Abstract
The direct inactivation of avian and murine oncoviruses by γ rays was examined using 60Co as a γ-ray source. The inactivation of murine leukemia virus (M-MuLV) followed single-hit kinetics with a target size comparable to the molecular weight of its genomic RNA. The subgroup D Schmidt-Ruppin strain of avian sarcoma virus (SR-RSV D) was more resistant to γ rays than M-MuLV and showed multihit inactivation kinetics with an extrapolation number of 5. The two viruses showed similar uv-inactivation kinetics, as previously reported, and were about 40–60 times more resistant to uv light than vesicular stomatitis virus. The reason for the difference in the effects of γ rays on MuLV and SR-RSV D was not elucidated. The genomic RNA of the SR-RSV D strain was degraded by γ irradiation faster than its infectivity, but viral clones isolated from the foci formed after γ irradiation had a complete genome. These results suggest that SR-RSV D has a strong repair function, possibly connected with reverse transcriptase activity.
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