Abstract
Summary The distribution and properties of DNA-dependent RNA polymerases separated by DEAE-Sephadex chromatography from extracts obtained after lysis of transplanted tumor nuclei were compared with those from rat spleen and kidney nuclei. The effect of α-amanitin has been investigated on the multiple forms of the enzyme isolated from normal and neoplastic extracts. The results show that while from rat spleen and kidney only two peaks containing polymerase activity were obtained, from the nuclei of four experimental tumors investigated, three distinct peaks were separated. On the basis of the degree of inhibition by α-amanitin, it was possible to classify the first two activities from tumor tissue as belonging to Class A and the third to Class B.
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