Abstract
The medicinal plants have been collected form wild resources and only some species used in larger quantities are cultivated systematically. Many medicinal plants, which were ignored in the past years, but the number of plants still formed in the wild is progressively declining the medicinal value with this situation to acquiring from the wild sources in more important recent trends bening. Reported in cresingly the antimicrobial properties of medicinal plants from different parts of the world .On this basis, the present investigation was focused on the screening of antimicrobial properties ofAsystasia indica Asystasia gangetica Thunbergia alata on selected Pathogenic bacteriaThe main objectives of this work use as follow To Screen the antimicrobial properties of these plants on selected micro organisms.To determine the effectiveness of inhibition on microbes by comparing its activity with known antibiotics.
Highlights
The antibacterial activity of six medicinal plants namely, Asystasia indica Asystasia gangetica Thunbergia alata against the tested microorganism (Salmonella typhi, Pseudom aeruginosa) were evaluated by the disc diffusion and streak plate method using different concentration of solvents. (Mazura and Norin 1993) (Rasooli, and M,r mostofa, (2002)(Puratchi et al 2001) The results showed significantly in all plants samples
The Asystasia gangetica, the results indicated that the leaf have shown better inhibition than other parts such as stem and root by each of the solvents
Antibacterial activity of Thunbergia alata showed better results in ethanolic leaf extract as well as stem than root Control plates were prepared for the comparison with the standard antibiotics namely ciprofloxacin
Summary
Plant materialThe following plants were collected from their natural habitats and used for their activity.Asystasia indica Asystasia gangetica Thunbergia alata at the College campus, Kodaikanal in Tamil Nadu.The plants were shade dried at ambient Temperature (31 C) and the dried materials were crushed into fine powder using an electric blender.Extract preparation100 g of dried powder materials of Leaf, Stem, Root, Tuber were soaked separately in 500 ml of each solvent viz. ethanol, chloroform and petroleum ether in conical flasks. The plants were shade dried at ambient Temperature (31 C) and the dried materials were crushed into fine powder using an electric blender. 100 g of dried powder materials of Leaf, Stem, Root, Tuber were soaked separately in 500 ml of each solvent viz. Each mixture was stirred at 24 hr using sterile glass rod. At the end of 72 hrs each of the extract was passed through the Whatman No.[1] filter paper and the filtrates were concentrated by opening the cover to 1 hr at room temperature in order to reduce the volume. Each of the extract was individually reconstituted using minimal amounts of the tracking solvent prior to use
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