Sensitivity of Phytophthora parasitica to mandipropamid: In vitro determination of baseline sensitivity and in vivo fungitoxicity

Abstract

Tobacco black shank caused by Phytophthora parasitica is a devastating soil-borne disease of tobacco in China. Control of tobacco black shank relies on numerous fungicide applications. A new carboxylic acid amide (CAA) fungicide, mandipropamid, was examined for its effects on various asexual developmental stages of P. parasitica in vitro and in tobacco seedlings. Although mandipropamid did not affect discharge of zoospores from sporangia, it strongly inhibited mycelial growth (mean EC50 value of 0.0112 μg ml−1), sporangia production (mean EC50 value of 0.009 μg ml−1), germination of encysted zoospores (mean EC50 value of 0.014 μg ml−1), and germination of sporangia (mean EC50 value of 0.017 μg ml−1). For protective activity in tobacco seedlings, various applications of mandipropamid were superior in reducing black shank compared to that of metalaxyl and of azoxystrobin; while for curative activity assay, 100 μg ml−1 of mandipropamid exhibited the same efficacy as that of metalaxyl, and presented superior activity than that of azoxystrobin. In 2010 and 2011, 119 isolates of P. parasitica from Guizhou Province of China were characterized for the baseline sensitivity to mandipropamid by measuring mycelial growth. Values of effective concentrations for 50% mycelia growth inhibition varied from 0.0068 to 0.0285 μg ml−1 and averaged 0.013 (±0.0045) μg ml−1, with a unimodal distribution. This information will serve as a baseline for tracking future changes in sensitivities of P. parasitica populations to mandipropamid in China.