Abstract
ABSTRACTGlutamic acid in cheese was determined by its reaction in a packed‐bed enzyme reactor containing immobilized glutamate dehydrogenase (GIDH) in a flow‐injection system, measuring fluorescence of NADH generated. There was a linear relationship (r = 0.999) between log‐peak height and log‐glumatic acid concentration (0.01 to 0.5 mM). The detection limit was 0.005 mM for an injection volume of 88 μX. The sample frequency was 30/h and the precision was better than 1.2% for 10 successive assays. The accuracy of the enzyme reactor‐flow injection system was evaluated by comparison with HPLC results and an excellent correlation was obtained (r = 0.996). No measurable decrease in activity of the GIDH column was observed after a 3‐month period.
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