Abstract
A stable, sensitive and low-reagent consumption method for the quantification of trace neurotoxin domoic acid (DA) was presented by pressurized capillary electrochromatography (pCEC) with laser-induced fluorescence (LIF) detection.
Highlights
Domoic acid (DA), the principal causative toxin of amnesic shell sh poisoning (ASP), is produced by the algae pseudonitzschia and could accumulate in the edible tissue of shell sh.[1,2] Consuming the contaminated shell sh seafoods could cause severe neurological symptoms such as memory loss, headaches, loss of balance, nausea, etc.[3]
Shell sh tissue), which was 130 times more sensitive than that of pressurized capillary electrochromatography (pCEC)-UV method (LOD, 2.0 mg DA per g). The sensitivity of this method would be better than that of capillary electrochromatography (CEC)-photodiode array detection (PAD), better than that of capillary electrophoresis (CE)-MS (70 ng DA per mL),[28] and kept in a similar level to normal HPLC methods for DA analysis (LOD, 6–25 ng DA per g).[7,8]. In this pCEC method, a small amount of mobile phase was used with the ow in capillary column of 1.2 mL minÀ1, which was only 1/800 of that used in normal HPLC
A stable and sensitive pCEC-laser-induced fluorescence (LIF) hyphenated method for the analysis of trace DA in shell shes was formed with low-reagent consumption, and the quanti cation for trace DA in shell sh seafoods was successfully achieved
Summary
Domoic acid (DA), the principal causative toxin of amnesic shell sh poisoning (ASP), is produced by the algae pseudonitzschia and could accumulate in the edible tissue of shell sh.[1,2] Consuming the contaminated shell sh seafoods could cause severe neurological symptoms such as memory loss, headaches, loss of balance, nausea, etc.[3]. Ow (EOF), and resulted in an efficient elution and the questions such as bubble formation and unstable quantitation could be effectively solved.[18,19,20] By superimposing a hydraulic pressure onto the electrically generated ow in capillary column, double modes that are electronic interaction and chromatographic retention could be employed for the efficient separation with a high resolution.[18,19] the mobile solution as low as nano-litre ow level in capillary column could be carried out facilely in pCEC It is desirable and become an attractive alternative to common HPLC and pure CEC for the robust and effective analysis. The recoveries of shell shes samples spiked with DA were analyzed, and the method validation in detection results was nally evaluated by comparing with the authoritative HPLC-MS/MS method
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