Abstract
A rapid and sensitive HPLC method with fluorescence detection has been developed for the micro-sample analysis of bisphenol A in rat serum. Compounds were separated on a Spherisorb C18 analytical column with acetonitrile-0.1% triethylamine in deionized water, 54:46 (v/v), as mobile phase. Bisphenol A was isolated by one-step extraction with dichloromethane. The calibration plot was linear (r=0.999) over the concentration range 1–1000 ng mL−1. The limits of detection and quantitation were 0.2 ng mL−1 and 1 ng mL−1, respectively, for 100-μL serum samples. The assay was successfully used for a pharmacokinetic study after intravenous administration of a low dose (0.1 mg kg−1) of bisphenol A to rats.
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