Abstract
The sensitive method for the simultaneous determination of eight atypical antipsychotic drugs (risperidone, olanzapine, quetiapine, clozapine, ziprasidone, perospirone, aripiprazole and blonanserin) in human serum has been developed based on a high-performance liquid chromatography-tandem mass spectrometry (LC/MS/MS) with electrospray ionization (ESI). The chromatographic separation was performed on a Mightysil-RP-18 MS column (2.0 mm × 150 mm, particle size 3 μm). The mobile phase consisted of 10 mM formic ammonium buffer (pH 6.0) and acetonitrile and was delivered at a flow rate of 0.20 mL/min. The triple quadrupole mass spectrometer was operated in positive ion mode, and multiple reaction monitoring was used for drug quantification. Solid-phase extraction of the eight antipsychotic drugs added to the human serum was performed with an Oasis®HLB extraction cartridges column. The method was linear for the investigated drugs over the concentration range of 20 - 100 ng/mL. The recoveries of these drugs were in the range of 81.3% - 140%. The intraday and inter day standard deviation (SD) values for all analytes were <0.10. Therefore, the selectivity, accuracy and precision of this method are sufficient for clinical and forensic studies. This simultaneous screening method will be also applicable to analyze other atypical antipsychotic drugs.
Highlights
The atypical antipsychotic drugs are one of the groups of antipsychotics used to treat psychiatric conditions
The sensitive method for the simultaneous determination of eight atypical antipsychotic drugs in human serum has been developed based on a high-performance liquid chromatography-tandem mass spectrometry (LC/MS/MS) with electrospray ionization (ESI)
In gas chromatography-mass spectrometry (GC/MS) analysis, it is necessary to gasify the sample and the analyzable samples are limited to relatively low molecular gas, volatile compound or thermally stable compound
Summary
The atypical antipsychotic drugs are one of the groups of antipsychotics used to treat psychiatric conditions. They are prescribed much more frequently and became a first-line agent for schizophrenia as they show less side-effect than the primarily used typical antipsychotic drugs [1]. These drugs are metabolized by the liver extensively and excreted into the urine in animals and humans. Fatal poisoning due to these drugs is often reported Because of their combination misuse or excessive dosage, various intoxication cases have been reported to date [2] [3] [4] [5]. It is important to develop a simple and sensitive way to analyze these drugs
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