Sensitive impedimetric biosensor for direct detection of diazinon based on lipases.

Nedjla Zehani,Nicole J Jaffrezic-Renault,Rochdi Kherrat,Sergei V Dzyadevych

doi:10.3389/fchem.2014.00044

Abstract

Two novel impedimetric biosensors for highly sensitive and rapid quantitative detection of diazinon in aqueous medium were developed using two types of lipase, from Candida Rugosa (microbial source) (CRL) and from porcine pancreas (animal source) (PPL) immobilized on functionalized gold electrode. Lipase is characterized to specifically catalyze the hydrolysis of ester functions leading to the transformation of diazinon into diethyl phosphorothioic acid (DETP) and 2-isopropyl-4-methyl-6-hydroxypyrimidine (IMHP). The developed biosensors both presented a wide range of linearity up to 50 μM with a detection limit of 10 nM for Candida Rugosa biosensor and 0.1 μM for porcine pancreas biosensor. A comparative study was carried out between the two biosensors and results showed higher efficiency of Candida Rugosa sensor. Moreover, it presented good accuracy and reproducibility, had very good storage and multiple use stability for 25 days when stored at 4°C.

Highlights

Chemicals released from agriculture or industry may potentially develop toxic effects in the environment and ecological systems
We find the lipase which it is an important enzyme in biological systems, where is catalyzes the hydrolysis of ester functions and the transformation of triacylglycerol to glycerol and fatty acids, it is a subclasses of esterases (Bhalchandra et al, 2008)
This paper describes a new biosensor system devoted for environmental application, based on impedimetric transduction incorporating two types of enzymes, lipase from Candida Rugosa CRL and lipase from porcine pancreas PPL for the detection of diazinon in aqueous medium

Summary

Introduction

Chemicals released from agriculture or industry may potentially develop toxic effects in the environment and ecological systems. Two novel impedimetric biosensors for highly sensitive and rapid quantitative detection of diazinon in aqueous medium were developed using two types of lipase, from Candida Rugosa (microbial source) (CRL) and from porcine pancreas (animal source) (PPL) immobilized on functionalized gold electrode.

Results

Conclusion