Abstract
Endogenous immunoreactive prolactin (PRL) in normal resting mammary gland and DMBA-induced mammary adenocarcinoma of female rats was demonstrated with the avidin-biotin-peroxidase complex (ABC) method. Positive staining obtained in the cytoplasm of glandular cells of the mammary gland were more intense on the apical borders of epithelial cells. The immunostaining in the induced tumors was variable, being often less than normal gland cells. The positivity could be seen only in the section fixed in Bouin's solution and treated with the ABC method using a rabbit antiserum against rat PRL. The immunostaining showed a strong positivity in the anterior pituitary tissue of the same animals, and it appeared specific for PRL because of its abolishment when the antiserum absorbed with rat PRL was used. The results reported here emphasize the value of ABC method in the Bouin's solution-fixed sections for detecting hormones in their target tissues.
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