Abstract
A gas chromatographic procedure using electron capture detection has been developed for the monosaccharide analysis fo submicrogram quantites of glycoproteins. Methanolysis of the glycorproteis and subsequent trifluoroacetylation of the resultant methyl glycosides using N-mehylbis(trifluoacetamide) is carried out in a single capillary tube. The carbohydrate composition of 0.1 μg of several glycoproteins has been quantitativly determined by this method.
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