Abstract
A fluorescence labelling reagent, 4-bromomethyl-6,7-methylenedioxycoumarin (BrMDC), was synthesized from sesamol and citric acid by Pechman condensation followed by bromination. Nanomole amounts of saturated aliphatic fatty acids were converted into the corresponding fluorogenic esters in the presence of anhydrous potassium carbonate and a crown ether as a catalyst and were separated by reversed-phase high-performance liquid chromatography (HPLC). Quantitative studies revealed that n-caproic acid was esterifted completely at low temperature and with sufficient reproducibility. The detection limit was just below 15 fmol per injection at a signal-to-noise ratio of 3. The fluorescence quenching of the BrMDC derivative was the lowest in conventional mixed solvent systems in comparison with those of two previously reported coumarin compounds. BrMDC was also applied to the simultaneous analysis of some acidic non-steroidal anti-inflammatry agents by reversed-phase HPLC.
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