Abstract
A normal-phase high-performance liquid chromatographic procedure is presented for the determination of robenidine following derivatization with dansyl chloride. The derivatization, separation and detection conditions were optimized. The dansyl derivative is first purified on a silica gel cartridge followed by HPLC analysis on a silica gel column. The eluate is monitored by fluorescence detection at 320 nm excitation and 485 nm emission wavelength. The lower limit of detection of robenidine is 0.4 μg/ml.
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