Abstract
A sensitive electrogenerated chemiluminescence (ECL) peptide-based biosensor was fabricated for the determination of troponin I (TnI) by employing gold nanoparticles as amplification platform. Two specific peptides including peptide1 with a sequence of CFYSHSFHENWPS and peptide2 with a sequence of FYSHSFHENWPSK were employed as capture peptide and report peptide, respectively. The peptide2 was labeled with ruthenium bis(2,2′-bipyridine) (2,2′-bipyridine-4,4′-dicarboxylic acid)-N-hydroxysuccinimide ester (Ru(bpy)2(dcbpy)NHS) at NH2-containing lysine via acylation reaction and utilized as the ECL probe. Gold nanoparticles were electrodeposited onto gold electrode and used as an amplification platform. The peptide-based biosensor was fabricated by self-assembling peptide1 onto the surface of gold nanoparticles-modified gold electrode through a thiol-containing cysteine at the end of the peptide1. When the biosensor reacted with target TnI, and then incubated with the ECL probe, a strong ECL response was electrochemically generated. The ECL intensity is directly proportional to the logarithm of the concentration of TnI in the range from 1 to 300 pg/mL. The biosensor employing gold nanoparticles as amplification platform shows high sensitivity for the detection of TnI with a detection limit of 0.4 pg/mL (S/N = 3). Moreover, the biosensor is successfully applied to analysis of TnI in human serum sample. This work demonstrates that the combination of a highly binding peptide with nanoparticle amplification is a great promising approach for the design of ECL biosensor.
Highlights
Acute myocardial infarction is a major cause of human death and responsible for one third of deaths in the world
We developed two homogeneous electrogenerated chemiluminescence (ECL) peptide-based methods for the determination of troponin I (TnI) using this peptide as a molecular recognition element [14, 15]
After gold nanoparticles were electrodeposited onto gold electrode, the peak current increased from 23.89 to 29.21 μA (Fig. S1 A, line b), ascribing to the increase of electrode surface area, which is confirmed by the CV results in 0.1 M H2SO4 (Fig. S1 B)
Summary
Acute myocardial infarction is a major cause of human death and responsible for one third of deaths in the world. We developed two homogeneous ECL peptide-based methods for the determination of TnI using this peptide as a molecular recognition element [14, 15]. The aim of this work is to develop a highly sensitive ECL peptide-based method for the determination of protein, on basis of the idea of encompassing gold nanoparticles as amplification platform and peptide as molecular recognition element.
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