Abstract
A nano-porous carbon electrode, fabricated in 0.1 mol L?1 NaOH by the electrochemical technique, was used for the electrochemical detection of dihydronicotinamide adenine dinucleotide, NADH, with an overpotential decrease of about 270 mV and a linear range from 1.0 ? 10?6 to 1.0 ? 10?4 mol L?1. Amperometric sensing of ethanol cooperating with alcohol dehydrogenase and nicotinamide adenine dinucleotide was successfully demonstrated. A linear response in the range from 5.0 ? 10?5 mol L?1 to 1.0 ? 10?2 mol L?1 was obtained, with a detection limit of 1.0 ? 10?5 mol L?1. The method was successfully employed to determine ethanol in beer with high precision.
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