Abstract
A novel and reliable direct electrochemical method was established for the detection of adenine, based on the differential pulse anodic stripping response at a poly(amidosulfonic acid) (poly-ASA)-modified glassy carbon electrode (GCE) fabricated by electropolymerization. The characterization of electrochemically synthesized poly-ASA film was investigated by atomic force microscopy, electrochemical impedance spectroscopy, and voltammetric methods. This poly-ASA-modified GCE could greatly enhance the detection sensitivity of adenine. At optimum conditions, the anodic peak exhibits a good linear concentration dependence in the range from 3.0 × 10−8 to 1.0 × 10−6 M (r = 0.9994). The detection limit is 8.0 × 10−9 M (S/N = 3). The proposed method could be used to determinate the adenine in tablets of vitamin B4 with satisfactory results.
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