Abstract
Free bilirubin, when present in excess in the human body, can cause a multitude of diseases and disorders and even be fatal; hence, detecting it is of paramount importance. Herein, we report a luminescence quenching-based non-enzymatic method for the convenient, reliable, and rapid detection of free bilirubin in blood serum samples using sensing films (nanosheets/PS, nanosheets-tta/PS, and nanosheets-dbt/PS) as luminescent sensors. The luminescence intensity of the sensing films is linearly related to the free bilirubin concentration. Nanosheets-tta/PS demonstrated excellent sensing properties for the sensitive and reliable detection of free bilirubin in the range of 0.0–60.0 μM with a correlation coefficient of 0.9915, as compared to nanosheets/PS or nanosheets-dbt/PS. The limit of detection for the determination of free bilirubin was 41 nM. This method can be used to design a sensor-based test spot as a medical detection device for the visual detection of free bilirubin.
Highlights
Bilirubin (BR) is a yellow metabolic breakdown product of normal blood and has important biological and diagnostic signi cance.[1,2] Free BR, known as BR IX, has a lipophilic nature and plays a signi cant role in the tissue uptake and toxicity of BR.[3]
When the BR IX concentration is greater than 20 mM, does the luminescence intensity of nanosheets/PS decrease with increasing BR IX concentration, which is not suitable for detecting the BR IX concentration in the normal range (6.0–17.1 mM)
The detection ranges of nanosheets-tta/PS (0.0–60 mM) and nanosheets-dbt/PS (0–200 mM) observed are suitable to detect the BR IX concentration in human serum samples
Summary
Bilirubin (BR) is a yellow metabolic breakdown product of normal blood and has important biological and diagnostic signi cance.[1,2] Free BR, known as BR IX, has a lipophilic nature and plays a signi cant role in the tissue uptake and toxicity of BR.[3] The normal concentration level of BR IX in human serum is less than 25 mmol LÀ1, which increases to >50 mmol LÀ1 in a jaundice-infected individual.[4,5,6] In addition to jaundice, excess BR IX can cause hepatitis, mental disorders, cerebral palsy, brain damage, and even death.[7,8,9] precise determination of the concentration of free BR is extremely important
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