Abstract
Populations of the bacterial wilt pathogen, Burkholderia caryophylli, in infected plants were estimated using our newly developed carnation seedling BIO-PCR assay, which combines pre-incubation in a selective medium and polymerase chain reaction. Bacterial density in leaves and in stems from each node was examined after injured roots on plants were inoculated. B. caryophylli was consistently isolated from the lower leaves from 5 to 35 days after inoculation; thus, leaves can be assayed without sacrificing parent stock. The BIO-PCR assay has higher specificity for closely related species than a conventional assay on the selective medium, and consistently detects B. caryophylli.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
