Abstract
A rapid and sensitive diagnostic system is essential for the on-site monitoring of sanitary conditions in the food industry. In this study, we developed a point-of-care platform for the detection of hygiene indicator bacteria, including E. coli, coliform, and total bacteria, using a portable fluorescence detector. Through the dual recognition process by PCR and CRISPR/Cas, our system enables the simultaneous detection of bacteria in three different groups with the high specificity. The optimal reaction conditions obtained by the systematic investigation achieved a detection sensitivity as low as 10-1 cells/μL (1 cell/reaction). Furthermore, spiking tests in real samples were successfully carried out, which demonstrated the utility of our portable system for food hygiene monitoring. The proposed system represents a key platform for the sensitive and specific detection of hygiene indicator bacteria in resource- or facility-limited areas.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
