Abstract
Using calcium carbonate nanoparticles as enzyme immobilization matrix, the developments of xanthine biosensor were achieved by both electrooxidation and electroreduction of the enzymatic-generated hydrogen peroxide based on xanthine oxidase (XnOx) and horseradish peroxidase (HRP). Amperometric detection of xanthine was evaluated by holding the modified electrode at 0.55 and −0.05 V (versus SCE), for XnOx/Nano-CaCO 3 and XnOx/HRP/Nano-CaCO 3, respectively. The linear dynamic ranges of anodic and cathodic detections of xanthine were between 2 × 10 −6 to 2.5 × 10 −4 M and 4 × 10 −7 to 5 × 10 −5 M, respectively. The detection limits were determined to be of 2 × 10 −6 and 1 × 10 −7 M with anodic and cathodic processes, respectively. At lower working potential, XnOx/HRP/Nano-CaCO 3/GCE bienzymatic system exhibited excellent selectivity; the bienzyme electrode was inert towards ascorbic and uric acid present. Moreover, the permeability of enzyme/Nano-CaCO 3 was evaluated by the use of rotating disk electrode voltammetry.
Published Version
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