Sensitive and rapid detection of bacterial endotoxin with a functional carbon nanotube field-effect transistor biosensor

Abstract

Endotoxin in the blood can cause unexplained fever, and even death due to endotoxemia and bacteremia. Rapid and sensitive detection of endotoxin has become a priority event to intervene in the occurrence of dangerous diseases in time. In this context, a carbon nanotubes-based field-effect transistor (CNTs FET) nanosensor is developed to realize the rapid, label-free and sensitive detection of endotoxin. The CNTs FET was fabricated by assembling the polymer-sorted high-purity semiconductor CNT films onto the sensing channel. In order to improve the detection sensitivity, carboxylated graphene quantum dots (cGQDs) were coupled to the CNT surface via the poly-l-lysine (PLL). After that, polymyxin B (PMB), which is highly specific for endotoxin, was covalently combined with cGQDs, thus enabling the capture and detection of endotoxin. The method not only displayed an extremely low level of limitation of detection in PBS (4.6 fg/mL) and serum (30.3 fg/mL), respectively, with superior resistance to interference, but also enabled the analysis of Gram-negative bacterial infections in blood samples in a short duration of time. Meanwhile, the receiver operating characteristic curve (ROC) proved the superior diagnostic accuracy of this method (AUC = 0.990). Considering its excellent performance, the constructed CNTs FET biosensor is a promising tool to provide early warning of disease for clinical Gram-negative bacteremia and endotoxemia.