Abstract
The oxidation of l-cysteine has been studied at a glassy carbon electrode by electrocatalytic effects of Nile blue A (NBA) as a mediator using cyclic, linear sweep voltammetry and chronoamperometry as diagnostic techniques. The results showed that the catalytic current of NBA depended on the concentration of l-cysteine. Although l-cysteine itself showed a very poor electrochemical response at the glassy carbon electrode, the response could be greatly enhanced by using NBA as a mediator, which enabled a sensitive determination of l-cysteine. This sensor could be used for determination of l-cysteine in the range of 10.0 × 10 −6 to 2.5 × 10 −4 M with a limit of detection of 1.3 μM. The rate constant was estimated for catalytic reaction of NBA with l-cysteine using chronoamperometry. The influence of potential interfering substances on the peak current was studied and the results showed that the method was highly selective for l-cysteine determination. The proposed method was used for the determination of l-cysteine in synthetic and real samples.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
