Abstract

Two sensors for the detection of the metal ion Cu(II), based on the same sensing layer, are compared. They rely on different transduction methods, i.e., electrochemistry and surface plasmon resonance (SPR). D,L-penicillamine was used as the specific receptor since it is a strong ligand for copper(II). Moreover it is easily immobilized on a gold layer by a self assembling procedure by contacting the gold layer overnight with water/EtOH (80/20) containing D,L-penicillamine, taking advantage of the spontaneous interaction of thiols with gold surfaces. Both the electrochemical and SPR platforms were derivatized in the same way. In the first case a gold disc was used as working electrode, and in the second one a thin gold layer (60 nm thick) was deposited by sputtering over the exposed core of a plastic optical fiber (POF), after removing the cladding along half circumference.

Highlights

  • In the present investigation, the possibility of using the same receptor for copper(II), D,L-penicillamine, in connection with different transduction methods for sensing purposes is examined

  • In the first case a gold disc was used as working electrode, and in the second one a thin gold layer (60 nm thick) was deposited by sputtering over the exposed core of a plastic optical fiber (POF), after removing the cladding along half circumference

  • The signal of analytical interest is the variation of the resonance wavelength when copper(II) binds to the ligand D,L-penicillamine fixed at the gold surface

Read more

Summary

Introduction

The possibility of using the same receptor for copper(II), D,L-penicillamine, in connection with different transduction methods for sensing purposes is examined. Sensors with electrochemical and SPR transduction are considered. In both these devices the D,L-penicillamine can be deposited over the transductor (gold surface chip) by formation of a self assembled monolayer (SAM). Chemical sensors are based on the strict integration of a receptor with an instrument able to generate a signal upon the combination of the receptor with the substrate. Both the characteristics of the binding reaction, in particular the affinity constant, and that of the transductor, for example the sensitivity, are of overwhelming relevance for determining the performance of a sensor. In this work we compare two different transduction methods, combined with the same receptor, D,L-penicillamine, for the detection of the metal ion Cu(II)

Sensing Platforms
Experimental Results
Conclusions
Full Text
Paper version not known

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call