Abstract
BackgroundSenna alata (S. alata) has numerous pharmacological activities including anti-lipogenic effect in high-fat diet (HFD)-induced obese mice. The present study investigated the effect of Senna alata (S. alata) leaf extracts on the regulation of abnormal glucose metabolism in HFD-induced obese mice.MethodsMale ICR mice were induced to become obese by being fed a HFD (45 kcal% lard fat) for 12 weeks. During the last 6 weeks of diet feeding, the obese mice were treated with the water extract of S. alata leaf at 250 and 500 mg/kg/day. After 6 weeks of treatment, blood was collected for measuring biochemical parameters. The liver, epididymal fat and skeletal muscle tissues were excised and kept for determining histology and western blot analysis.ResultsTreatment with S. alata (250 and 500 mg/kg) significantly reduced hyperglycemia, hyperinsulinemia, and hyperleptinemia. The glucose intolerance was improved by S. alata. The elevated monocyte chemoattractant protein-1 (MCP-1) and tumor necrosis factor-α (TNF-α) levels in obese mice were reduced in S. alata treatment. The level of serum adiponectin was increased in obese mice treated with S. alata (250 and 500 mg/kg). The epididymal fat weight was reduced in S. alata treatment. The enlarged adipocyte size was smaller in obese mice treated with S. alata. In comparison with the obese control mice, the mice treated with S. alata showed a significant reduction of liver glucose-6-phosphatase (G6Pase) and phosphoenolpyruvate carboxykinase (PEPCK) proteins. Moreover, S. alata up-regulated the liver and muscle adenosine monophosphate-activated protein kinase phosphorylation (pAMPK) and muscle glucose transporter 4 (GLUT4).ConclusionsThe results indicate that the restoration of impaired glucose metabolism of S. alata may be associated with reduced hepatic gluconeogenesis and increased glucose uptake via AMPK activation.
Highlights
Senna alata (S. alata) has numerous pharmacological activities including anti-lipogenic effect in highfat diet (HFD)-induced obese mice
The results indicate that the restoration of impaired glucose metabolism of S. alata may be associated with reduced hepatic gluconeogenesis and increased glucose uptake via adenosine monophosphateactivated protein kinase (AMPK) activation
The present study investigated the effect of S. alata on impaired glucose metabolism using high-fat diet (HFD)-induced obese mice and measuring the parameters associated with hepatic glucose production, AMPK phosphorylation, and glucose transporter 4 (GLUT4) translocation
Summary
Senna alata (S. alata) has numerous pharmacological activities including anti-lipogenic effect in highfat diet (HFD)-induced obese mice. The present study investigated the effect of Senna alata (S. alata) leaf extracts on the regulation of abnormal glucose metabolism in HFD-induced obese mice. Obesity is a condition that is strongly associated with insulin resistance and diabetes. In studies using obesity-related insulin resistance models, the up-regulation of hepatic. In mice fed with high-fat diet (HFD), absence of AMPKα2 in skeletal muscle subunit led to a development of glucose intolerance and insulin resistance [6]. Activation of AMPK in the liver and muscle is expected to elicit beneficial metabolic effects with the potential to improve the defects associated with T2DM and the metabolic syndrome [8]
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.