Abstract
The changes of Zn 2+ -dependent acid inorganic pyrophosphatase (Zn 2+ -IPPase) activity in detached and intact leaves of rice seedlings ( Oryza sativa L. cv. Taichung Native 1) during senescence were investigated. Zn 2+ -IPPase activities in freshly excised leaves and excised leaves that had been incubated in darkness for 4 days were monitored with a pH optimum of 6.2. Calcium significantly inhibited Zn 2+ -IPPase activity. Zn 2+ -IPPase was present in detached and intact leaves throughout senescence. There seems to be a direct correlation between senescence and decrease of Zn 2+ -IPPase activity in detached leaves, but not in intact leaves. It seems unlikely that Zn 2+ -IPPase and Mg 2+ -IPPase activities are due to the same enzyme protein.
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