Senescence is delayed by selenium in oilseed rape plants

Abstract

Leaf senescence is a genetically programmed process that can also be induced by nitrogen (N) deficiency. Although selenium (Se) delays leaf senescence, the underlying mechanisms are still unknown. To explore the mechanisms of Se-mediated delay of leaf senescence, we studied the biochemical and molecular events that occur during developmental and N deficiency-induced senescence. Oilseed rape (Brassica napus L.) plants were grown under adequate N (AN, 16 mM) or low N (LN, 4 mM) conditions during the rosette growth stage and treated with Se (15 μg plant–1 as Na2SeO4) either through roots or leaves for four weeks. Shoot dry matter production was not influenced, while the photosynthetic parameters were improved by Se application in both young and old leaves under both AN and LN conditions. The Se treatment rarely influenced the concentrations of reactive oxygen species (ROS), while it increased the nitric oxide (NO) levels in young and old leaves under both AN and LN conditions. The positive correlation between the NO level and leaf photosynthetic parameters in old leaves of LN plants suggested a role for NO boosting, mediated by Se, in the protection of aging leaves from LN-induced accelerated senescence. This implication was further supported by the clear down-regulation of SAG12-1 and up-regulation of Cab, particularly by root application of Se in old leaves of LN plants. Our results provide the first evidence that Se influences the expression of senescence-associated genes and delays senescence through NO signalling but is independent of the ROS defence system.