Abstract
A decline in nitrogenase activity (C2H2 reduction) of nodules of Phaseolus vulgaris L. cv. Contander was correlated with a decrease in their soluble protein including leghe‐moglobin. Concomitantly, two distinct proteolytic activities against leghemoglobin with acidic and alkaline pH optima were detected. The corresponding proteases were purified about 30‐fold by ammonium sulfate precipitation, gel filtration and hydroxy‐apatite chromatography. Both the acidic (pH optimum 3.5) and the alkaline (pH optimum 8.0) proteases were thiol enzymes. They were characteristic of senescing nodules, whereas only an acidic serine protease was present in functional nodules.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
