Abstract
Senecavirus A (SVA), classified into the genus Senecavirus in the family Picornaviridae, causes an infectious disease in pigs. This virus can efficiently replicate in some non-pig-derived cells, such as the BHK cell line and its derivative (BSR-T7/5 cell line). We had recovered a wild-type SVA from its cDNA clone previously, and then uncovered the proteomic profile of SVA-infected BSR-T7/5 cells at 12 h post inoculation (hpi). In order to explore the cellular metabolomics further, the SVA-inoculated BSR-T7/5 cell monolayer was collected at 12 hpi for assay via liquid chromatography-tandem mass spectrometry (LC-MS/MS). The resultant data set was comprehensively analyzed using bioinformatics tools. A total of 451 metabolites were identified using in-house and public databases. Out of these metabolites, sixty-one showed significantly differential values (p value < 0.05). The Kyoto Encyclopedia of Genes and Genomes (KEGG) database was used to analyze metabolic pathways of the significantly differential metabolites. There were eighty-one identified KEGG pathways, out of which twenty-seven showed their p values < 0.05. The pyrimidine metabolism revealed the minimum p value and the maximum number of significantly differential metabolites, implying the pyrimidine played a key role in cellular metabolism after SVA infection. SVA replication must rely on the cellular metabolism. The present study on metabolomics would shed light on impacts of SVA-induced multiple interactions among metabolites on cells or even on natural hosts.
Highlights
Senecavirus A (SVA), formerly known as Seneca Valley virus, is an emerging virus that causes vesicular disease and epidemic transient neonatal losses in pigs
We found that the cell-adapted SVA could induce a robust cytopathic effect (CPE) on a BSR-T7/5 cell monolayer at 24 h post inoculation or even earlier (Liu et al, 2021a)
Total ion chromatograms (TICs) of quality control (QC) samples were compared with one another in positive (Figure 2A) and negative (Figure 2B) ion modes, showing highly overlapping response intensity and peak retention time
Summary
Senecavirus A (SVA), formerly known as Seneca Valley virus, is an emerging virus that causes vesicular disease and epidemic transient neonatal losses in pigs. This virus belongs to the genus Senecavirus in the family Picornaviridae. It is the only member in the genus Senecavirus. Mature virion is a non-enveloped icosahedral particle with a diameter of approximately 27 nm. The viral genome is a positive-sense, single-stranded and nonsegmented RNA, approximately 7300 nucleotides (nt) in length, with a 3’ poly (A) tail but without a 5’ capped structure. The genome contains 5’ and 3’ untranslated regions, and a single long open reading frame (ORF) of polyprotein
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