Abstract
The normal replication of Semliki Forest virus involves RNA synthesis followed by the synthesis of two structural proteins (nucleocapsid and membrane). Temperature-sensitive mutants blocked at different steps of the growth cycle have been isolated from Semliki Forest virus by chemical mutagenesis. A group of the mutants defective in RNA synthesis (RNA − mutants) were prevented from producing viral antigen in cells infected at the restrictive temperature and also were unable to inhibit host cell RNA synthesis. The factor affecting viral RNA synthesis is expressed early in the growth cycle. Other mutants were defective in functions subsequent to viral RNA synthesis (RNA + mutants). Some were defective in the production of nucleocapsids only; others failed to make membrane only. A few RNA + mutants made both structural proteins, but not infectious virus, and may be defective in a maturation function.
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