Abstract
TRODAT-1 is a radiopharmaceutical derived from tropane and linked to tecnecium-99m ([99mTc] TRODAT-1) has been used in studies of dopamine transporter (DAT) in central nervous system. Associated with the SPECT technique of acquisition, is able to detect changes in neurological disorders like Parkinson´s disease, evaluating the binding potential (BP) of DAT. The aim of this study was to evaluate the influence of the image reconstruction methods, Filtered Back Projection (FBP) and iterative reconstruction (OSEM), in BP values at the striatal region in 30 healthy volunteers. Images were analyzed by visual inspection and semi-quantitative analysis. Regions of interest (ROI) were made over striatal areas on both sides. Nonparametric Wilcoxon statistical analysis was performed between the BP values from the FBP and OSEM methods. Our results showed that the reconstruction methods have a statistical significant BP values difference in the total striatum (Z = -2,2787 p = 0.005), right striatum (Z = -2,602 p = 0.009) and left striatum (Z= 2,746 p = 0.006). The effect size was calculated to see if there influence in this test: the "large effect size" for all measurements was observed (total striatum r= -0.51; right striatum r= -0.48; left striatum r= -0.50). FBP is the usual method of reconstruction for brain SPECT images, and our results showed influence of the OSEM method in BP. It is concluded that the method of image reconstruction adopted should be standardized to avoid incorrect evaluations of BP values using [99mTc]TRODAT-1.
Highlights
Dopamine (DA) is a catecholamine that participates in motor, emotional, and cognitive functions
Filtered Back Projection (FBP) is the usual method of reconstruction for brain Single Photon Emission Computed Tomography (SPECT) images, and our results showed influence of the OSEM method in binding potential (BP)
Seventy percent of the DA removed from striatal synaptic clefts is mediated by DA transporter (DAT), regulating the local levels of available DA, and its concentration reflects the homeostatic tone of the dopaminergic system [4]
Summary
Dopamine (DA) is a catecholamine that participates in motor, emotional, and cognitive functions. Is the most important neurotransmitter of the central nervous system (CNS) [1,2]. There are different DA receptors at synaptic cleft that interact with this neurotransmitter. The DA transporter (DAT) is a molecule in the presynaptic neuron that modulates the uptake of DA [3]. Seventy percent of the DA removed from striatal synaptic clefts is mediated by DAT, regulating the local levels of available DA, and its concentration reflects the homeostatic tone of the dopaminergic system [4]. Alterations in dopaminergic system can lead to neurologic and neuropsychiatry disorders. Parkinson's disease (PD) is a common neurodegenerative disorder characterized by progressive degeneration of dopaminergic neurons in the substantia nigra, with loss of their nerve terminals in the basal ganglia structures, especially in the striatum [5]. PD diagnosis is based on clinical symptoms and is characterized by resting tremor, rigidity, bradykinesia, and postural instability [5,7]
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