Semi-quantitative analysis on the content of berberine hydrochloride in compound berberine tablets with the fluorescence spectral imaging method

Lan Liang,Qing He,Zhenqiang Chen,Lin Lin,Jinyan Sun,Siqi Zhu

doi:10.1142/s1793545816500188

Abstract

The content of berberine hydrochloride (BH) in compound berberine tablets (CBTs) is subject to strict requirements. Its content is usually measured based on chemical analysis. In this paper, the fluorescence spectral imaging method was used to study the relative content of BH from a physics perspective. By comparing the relative fluorescence intensity of self-made CBTs with different mass percentages of BH, a linear positive relationship was observed between the BH content and the relative fluorescence intensity, and accordingly the quality of CBTs of different brands was evaluated. The results indicate that the fluorescence spectral imaging method can be a simple, fast and nondestructive semi-quantitative analysis method to determine the content of BH in CBTs, and this method has great potential in the quality control of CBTs.

Highlights

The compound berberine tablet (CBT) is a kind of Chinese patent medicine (CPM)
The curve shape and peak-to-valley position of the spectrum curves showed that the °uorescence spectrum curves for radix paeoniae alba, radix aucklandiae, fructus evodiae and berberine hydrochloride (BH) were very similar to their standard reference materials, suggesting that the high quality of these four Chinese medicinal materials has guaranteed the quality of the self-made CBT.[14,15]
The content of the BH in the self-made CBT was semi-quantied by the °uorescence spectral imaging method

Summary

Introduction

The compound berberine tablet (CBT) is a kind of Chinese patent medicine (CPM). It is composed of berberine hydrochloride (BH), radix aucklandiae, fructus evodiae, radix paeoniae alba. Its e®ects include cleaning heat, drying humidity, promoting qi circulation, relieving pain and curing durchfall. It is mostly used to treat diseases like damp-heat in the large bowel, diarrhea and tenesmus. BH is the antibacterial ingredient in CBT, and the daily recommended dosage is less than 0.9 g. According to the Chinese pharmacopoei (2010), CBT (0.5 g)

Methods

Results

Conclusion