Abstract
Abstract terile rooms or reach-in boxes were used to prevent contamination of cultures in early work with plant tissue and embryo culture. The new sterile hoods, which are a spinoff of the aerospace program (2), make continuous work at transferring tissue cultures comfortable and reduce contamination. The laminar flow hood, evaluated by Coriell and McGarrity (1), is particularly comfortable and effective. These hoods are large, heavy, and expensive pieces of equipment and not entirely suitable for protoplast manipulation and other microscope work. This report will describe a sterile hood, which can be built easily with readily obtainable components and has advantages over many of the available commercial units. The laminar flow hood described is reasonably portable and minimizes vibration of the work surface, making it ideal for microscope work.
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